Publications

2024

R. Tapia-Rojo, M. Mora, S. Garcia-Manyes. “Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically relevant forces and timescales”, Nature Protocols (2024) Link
R. Tapia-Rojo, Alonso-Caballero, CL Badilla, JM Fernandez, “Identical Sequences, Different Behaviors: Protein Diversity Captured at the Single-Molecule Level” Biophysical Journal, 123, 814-23 (2024) Link
R. Tapia-Rojo. “Construction and operation of a high-resolution magnetic tape head tweezers for measuring single-protein dynamics under force”, Methods in Enzymology (Elsevier, Book Chapter) 694, 83-107 (2024) Link
J Darrigrand, A. Salowka, A. Torres-Cano, R. Tapia-Rojo, T. Zhu, S. Garcia-Manyes, F. M. Spagnoli. “Acinar-ductal cell rearrangement drives branching morphogenesis of the murine pancreas in an IGF/PI3K-dependent manner” Developmental Cell, 59, 326-38. (2024). Link

2023

M. Mora, R. Tapia-Rojo, S. Garcia-Manyes, “Unfolding and Refolding Proteins Using Single-Molecule AFM”, Single Molecule Analysis: Methods and Protocols, (Springer US, Book Chapter) 339-354, (2023). Link
M. Bernabe-Rubio, S. Ali, P. Bhosale, G. Goss, A.S. Mobasseri, R. Tapia-Rojo, T. Zhu, M. Battilocchi, I. Tomas, C. Ganier, S. Garcia-Manyes, F. Watt, “Mechanical factors induce Myc-dependent dedifferentiation of adult epidermal cells via direct reversal of the terminal differentiation trajectory” Nature Cell Biology, 25, 1426–1438, (2023). Link
F. Franz*, R. Tapia-Rojo*, S. Winograd-Katz, R. Boujemaa-Paterski, W. Li, T. Unger, S. Albeck, C. Aponte-Santamaria, S. Garcia-Manyes, O. Medalia, B. Geiger, F. Grater, F. “Allosteric activation of vinculin by talin”, Nature Communications, 14:4311, (2023). Link
R. Tapia-Rojo*, M. Mora*, S.J. Board, J. Walker, R. Boujemaa-Paterski, O. Medalia, S. Garcia-Manyes, “Enhanced statistical sampling reveals microscopic complexity in the talin mechanosensor folding energy landscape”, Nature Physics, 19, 52-60, (2023). Link

2020

A. Alonso-Caballero*, D.J. Echelman*, R. Tapia-Rojo, S. Haldar, E.C. Eckels, et al “Protein folding modulates the chemical reactivity of a Gram-positive adhesin” Nature Chemistry, 13, 172-181, (2020). Link
R. Tapia-Rojo, A Alonso-Caballero, J.M. Fernandez. “Talin folding as the tuning fork of cellular mechanotransduction” Proceedings of the National Academy of Sciences, 117, 21346-21353, (2020). Link
R. Tapia-Rojo, A. Alonso-Caballero, J.M. Fernandez, “Direct observation of a coil-to-helix contraction triggered by vinculin binding to talin” Science Advances, 6, aaz4707, (2020). Link
J.A. Rivas-Pardo, Y. Li, Z. Mártonfalvi, R. Tapia-Rojo, A. Unger, et al, “A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues” Nature Communications, 11, 2060, (2020). Link

2019

R. Tapia-Rojo, J.J. Mazo, F. Falo, Thermal versus mechanical unfolding in a model protein” The Journal of Chemical Physics, 151, 185105, (2019). Link
E.C. Eckels, S. Haldar, R. Tapia-Rojo, J.A. Rivas-Pardo, J.M. Fernandez, “The mechanical power of titin folding” Cell Reports, 27 (6), 1836-1847. e4, (2019). Link
R. Tapia-Rojo, E.C. Eckels, J.M. Fernandez, “Ephemeral states in protein folding under force captured with a magnetic tweezers design” Proceedings of the National Academy of Sciences, 116, 7873-7878, (2019). Link

2018

J.A. Rivas-Pardo, C.L. Badilla, R. Tapia-Rojo, A. Alonso-Caballero, J. M Fernandez, “Molecular strategy for blocking isopeptide bond formation in nascent pilin proteins” Proceedings of the National Academy of Sciences, 115, 9222-9227, (2018). Link
E.C. Eckels, R. Tapia-Rojo, J. A. Rivas-Pardo, J. M Fernández. “The work of titin protein folding as a major driver in muscle contraction”, Annual review of physiology, 80, 327-351, (2018). Link

2017

S. Haldar, R. Tapia-Rojo, E. C. Eckels, J. Valle-Orero, J. M Fernandez, “Trigger factor chaperone acts as a mechanical foldase”, Nature Communications, 8, 668 (2017). Link